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National Institute for Genomics & Advanced Biotechnology (NIGAB)
NATIONAL AGRICULTURAL RESEARCH CENTRE, PARK ROAD ISLAMABAD-45500 |
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Home Page • Plant Biotechnology • Animal Biotechnology
Training workshops curriculum(View full calender)
Training I
One day Hands-on Workshop on "Latest Bioinformatics Intervention in Agrigenomics and Proteomics" (17th December, 2019)
Contents overview
- NGS Data Analysis, Quality check, transriptome assembly, Differential expression and enrichment analyses by Ballgown/R (Practical)
- Agricultural phenotypic, traits and molecular data analysis using R and advanced graphics (Practical)
- Structural Bioinformatics, Homology modeling and drug discovery (Practical)
Registeration fee:
- For students: 4000 Rs.
- For faculty and others: 5000 Rs.
Contact persons:
- Dr. Muhammad Ramzan Khan:
Cell: 0332-5394396
E-mail: drmrkhan_nigab@yahoo.com - Muhammad Aqeel:
Cell: 0321-4786570
E-mail: muhammadaqeel24@gmail.com - Wajya Ajmal:
Cell: 0333-5661196
E-mail: wajya.ajmal@yahoo.com
Training II
Two days Training Workshop on "Microbial Identification based on Advanced Molecular Techniques" (18-19th February, 2020)
Contents overview
- Serial Dilution and Culturing, Gram Staining and Catalase (Practical)
- DNA Extraction and PCR, Gel Electrophoresis, Gel Doc (Practical)
- Preparation of Probiotics (Practical)
- Strain Identification by Blast, Advance Query Genus 16S Extraction,
Multiple Sequence Alignments, Phylogenetic Tree Building (Practical)
Registeration fee:
- For students: 4000 Rs.
- For faculty and others: 5000 Rs.
Contact person:
- Dr. Shakira Ghazanfar:
Cell: 0332-5554517
E-mail: shakira_akmal@yahoo.com
Training III
Two days Training Workshop on "Marker assisted Breeding for Crop Improvement" (14-15th April, 2020)
Contents overview
- DNA markers and their utilization in crop improvement, PCR Based DNA Markers
- DNA Extraction and Quantification (Practical)
- Microsatellites DNA Markers, Simple Sequence Repeats (SSRs), Inter Simple Sequence Repeats (ISSRs),
Designing of primers, Automation in SSR /ISSR analysis (Practical)
Registeration fee:
- For students: 4000 Rs.
- For faculty and others: 5000 Rs.
Contact persons:
- Dr. Armaghan Shahzad:
Cell: 0334-9585395
E-mail: armghan_shehzad@yahoo.com - Mrs. Sania Begum:
Cell: 0334-8252487
E-mail: sania.idrees@gmail.com
Training IV
Two days Training Workshop on "Basic Laboratory Analysis of Genetically Modified Crops" (19th June, 2020)
Contents overview
- GM crop status in Pakistan
- National and International status of GM crops
- National and International Accords for GM crops
- Genetic transformation of crop plants
- GMO Detection Methods
- Nucleic Acid based detection of GM crops (Practical)
- Protein based methods of GMO Detection (Practical)
Registeration fee:
- For students: 4000 Rs.
- For faculty and others and others: 5000 Rs.
Contact persons:
- Dr. Shaukat Ali:
Cell: 0321-6623253
E-mail: shaukat_parc@yahoo.co.in - Muhammad Amir Zia:
Cell: 0314-9881118
E-mail: amirzia_narc@yahoo.com
Training V
Two days Training Workshop on "Gene Cloning and its Applications" (15-16th September, 2020)
Contents overview
- Introduction to gene cloning and its applications
- Isolation of RNA, Quality assessment and Quantification (Practical)
- Primer designing (Practical)
- cDNA Synthesis by RT-PCR/ gene amplification, TA/ Cloning
- Gene Editing
- An overview of Genome Editing, Single Guide RNA Designing Tools (Practical)
- Plasmid extraction by Mini Prep, Gene Delivery Vector construction (Practical)
Registeration fee:
- For students: 4000 Rs.
- For faculty and others: 5000 Rs.
Contact persons:
- Dr. Muhammad Ramzan Khan:
Cell: 0332-5394396
E-mail: drmrkhan_nigab@yahoo.com - Dr. Nazia Rehman:
Cell: 0333-5073013
E-mail: naziarehman96@yahoo.com
Training VI
Two days Training Workshop on "Potato Tissue Culture" (17-18th November, 2020)
Contents overview
- Importance of potato tissue culture and Media Preparation (MS 1962) for Potato & sterilization
- Preparation of plant material and Meristem culture and In vitro Multiplication of Potato (Practical)
- Virus indexing and Nucleus seed production (Practical)
Registeration fee:
- For students: 4000 Rs.
- For faculty and others: 5000 Rs.
Contact persons:
- Dr. Aish Muhammad:
Cell: 0302-5036051
E-mail: aish.muhammad@yahoo.com - Dr. Kazim Ali:
Cell: 0333-6739099
E-mail: kazim76@gmail.com - Muhammad Zeeshan:
Cell: 0301-5556695
E-mail: muhammadzeeshan95@gmail.com
Doctor of Philosophy (PhD)
| Shahdana Zia |
| Sahir Hameed Khatak |
| Sania Begum |
| Anila |
Master of Philosophy (M Phil)
| Muhammad Rizwan |
| Muhammad Ehtsham |
| Syeda Tayyeba |
PLANT BIOTECHNOLOGY CLUSTER
Gene Isolation
Achievements
Important isolated genes:
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Gene Transformation
Achievements
High Yielding Genetically Engineered Crops
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Marker Assisted Selection
Achievements
Stress Tolerant Crops with (7 to 12%) more Yield
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Tissue Culture
Achievements
High Yielding Disease Free Banana:
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ANIMAL BIOTECHNOLOGY CLUSTER
Animal Genomics
Achievements
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Vaccine Development
Achievements
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Probiotics Development
Achievements
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FMDV Characterization
AchievementsAchievements are:
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Disease Diagnosis
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BIOINFORMATICS CLUSTER
Marker-Trait Association
Marker-Trait AssociationEstablished the statistical modeling protocols for association-building between molecular markers and economic traits See more... |
Drug Designing
Computational BiologyOptimized CPI vigenettes in R for Protein-ProteinInteraction Studies Optimized Bio3D vigenettes for Protein analyses See more... |
System Biology
Gene EnrichmentOptimized various R packages for building classes of Co-regulated Biological Processes using Differentially Expressed Genes See more... |
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Scientific Staff
ORCID
Administrative Staff
Other Staff
State of the Art Research Labs
Plant Biotechnology, NIGAB, NARC Islamabad
Email: sania.idrees@gmail.comPhone: 0092-51-90733827/8 Research Activities:
- Marker Assisted Selection (MAS) for development of new wheat varieties for rust, karnal bunt, salinity and drought tolerance
- Genetic diversity and phytochemical analysis of different indigenous accessions of crop plants
- Identification of genome for pathogenicity related genes and validation using newfangled SSR markers
- Identification of unknown olive varieties and adulteration in exportable commodities for quality assurance through DNA fingerprinting
Plant Biotechnology, NIGAB, NARC Islamabad
Email: zaheerpbg@yahoo.comPhone: 0092-51-90733815/6 Research Activities:
- Optimization of regeneration and transformation protocols for different crops (maize, wheat, potato, tomato, sugarcane, banana and groundnut)
- Development of genetically modified crops for economically important traits
- Molecular, biochemical and morphological evaluation of transgenic crops
- Molecular based diagnostic testing of GMOs
- Agrobacterium mediated genetic transformation of wheat, maize, potato and tomato for biotic and abiotic resistance
Plant Biotechnology, NIGAB, NARC Islamabad
Email: naziarehman96@yahoo.comPhone: 0092-51-90733819 Research Activities:
- Isolation of agricultural important genes from various plant species for environmental stress tolerance and enhancement in quality traits
- Structural characterization of new isolated genes by different bioinformatics tools
- Functional characterization of novel genes in model plants
Plant Biotechnology, NIGAB, NARC Islamabad
Email: amirzia_narc@yahoo.comPhone: 0092-51-90733824 Research Activities:
- Detection of Bt genes in cotton
- Quantification of Bt gene in cotton cultivars
- Genetic diversity study in cotton
- Genetic transformation and expression analysis study in tomato and chick pea
- Testing of commercial transgenic events of maize
Plant Biotechnology, NIGAB, NARC Islamabad
Email: kazim76@gmail.comPhone: 0092-51-90733822 Research Activities:
- Production of true to type and disease free plants of vegetatively propagated crops on mass scale for dissemination and to provide initial explant for genetic transformation
- Coordination at National and International level and capacity building of manpower in plant tissue culture
Plant Biotechnology, NIGAB, NARC Islamabad
Email: drmrkhan_nigab@yahoo.comPhone: 0092-51-90733808 Research Activities:
- Genome editing using CRISPR-Cas9/dCas9/CPf1 approaches for enhancement of plant productivity
- Next Generation Sequencing data obtained through genome re-sequencing and transcriptome (RNA-seq) sequencing is being analyzed for varietal/hybrid identification, SNP markers development and gene discovery
- Novel isolated genes are used for genome editing in plants
Bioinformatics, NIGAB, NARC Islamabad
Email: muhammadaqeel24@gmail.comPhone: 0092-51-90733812 Research Activities:
- Comparative analysis, multiple alignment and phylogenetic analysis of sequences
- Analysis of post-sequencing data and gene association studies
- Integration/ incorporation/ re-equipment of new bio-informative tools and software in lab
- In-silico drug designing and system biology of putative co-regulated biological process analyses using R
Animal Biotechnology, NIGAB, NARC Islamabad
Email: naeem_nibge@yahoo.comPhone: 0092-51-90733842 Research Activities:
- Genetic improvement of livestock breeds using advanced Molecular techniques
- DNA fingerprinting of milk related genes through various genomic tools
- Molecular Diagnostics & therapeutics
Animal Biotechnology, NIGAB, NARC Islamabad
Email: tahirakhurram3@gmail.comPhone: 0092-51-90733837 Research Activities:
- Standardization of LAMP-PCR for rapid detection of Foot and Mouth Disease in cattle
- Development of Monoclonal antibodies against Peste Des Pestitis Ruminants (PPR)
- Identification of antibiotic resistance genes in E.coli from meat samples
- Poultry disease diagnosis through clinical signs, laboratory tests and prescription of treatment
- Maintenance of cell lines and virus bank of local poultry viral isolates
- Standardized of chicken primary cell culture protocols for ISO certification
Animal Biotechnology, NIGAB, NARC Islamabad
Email: shakira_akmal@yahoo.comPhone: 0092-51-90733833 Research Activities:
- Identification of economically beneficial indigenous species of microorganisms based on 16S rRNA gene sequence
- Imaging of bacterial cells (phase-contrast microscopy/fluorescent microscopy)
- Physiological and Biochemical characterization analysis of bacteria using API kits
- To establish a long term microbial preservation facility as "NIGAB Microbial Collection of Pakistan (NMCCP)
- To collaborate with different microbial culture collection for exchange of strains for research and development.
- To function as a depository of microorganisms involved in patent procedures.
Animal Biotechnology, NIGAB, NARC Islamabad
Email: aatka.214@gmail.comPhone: 0092-51-90733833 Research Activities:
- Veterinary Sciences, Animal biotechnology
- Molecular Epidemiology, Diagnostic of Animal Diseases
- and Vaccine Production
Animal Biotechnology, NIGAB, NARC Islamabad
Email: naeem_nibge@yahoo.comPhone: 0092-51-90733827 Research Activities:
- Biological evaluation of pathogens
- Raising hyper immune serum
- Raising antigens
- Animal vaccine trials
- Biosafety studies
- Virus growth
Animal Biotechnology, NIGAB, NARC Islamabad
Email: ahmad.ali616@gmail.comPhone: 0092-51-90733839 Research Activities:
- Genetic Polymorphism association with economic traits
- Isolation and Visualization of Human, Viral and bacterial Nucleic Acids from different biological specimens
- Nucleic acid quantification by Real Time Polymerase Chain Reaction (PCR)
- Restriction Fragment Length Polymorphism (RFLP)
Products
High yeilding banana plants
- NIGAB-1
- NIGAB-2
- NIGAB-3
- NIGAB-4
Commercial Services
- Testing of GMOs for trade commodities
- DNA bar-coding of varieties for Plant Breeder Rights registration
- Bt gene authentication and expression quantification in cotton for NCVT
- Basmati and Non Basmati Rice adulteration
Research Facilities
- DNA fingerprinting for variety identification (Olive plants)
- Sex determination in papaya through DNA markers
- DNA diagnostics for FMDV and PPRV
- Genetic testing of dairy milk protein
- Bio-safety risk assessment for GMOs
- Next generation sequencing (NGS) data analyses facility
- Bio-safety cabinet III (BSL III) facility
- Animal containment facility
- Cell culture room
- DNA Extraction and quantification
| Project title | Funding source | Worth |
|---|---|---|
| Molecular marker based identification of halal and non-halal meat type | PSF | 4.00 MRs. |
| Development of Indigenous Probiotic Feed Additive Products to Improve Productivity of Dairy Buffalo in Cost Effective Manners | PARB | 19.86 MRs. |
| Yield enhancement of wheat through improved grain number and spikelet architecture using translational genomics and genome editing approaches | ALP | 7.40 MRs. |
| Comparative genomics of wheat and desiccation-tolerant Oropetium grass for root architecture variations, and CRISPR-Cas9/dCas9-mediated genome editing of root growth genes in wheat for more wheat to Pakistan | ALP | 7.00 MRs. |
| Project title | Funding source | Worth |
|---|---|---|
| National Institute for Genomics and Advance Biotechnology | PSDP | 482.701 MRs. |
| Genomic studies for stress tolerance in wheat and rice | RADP | 71.785 MRSs. |
| Development of Indigenous cotton Hybrid seed | RADP | 109.1 MRs. |
| Cotton Hybrid seed Production | RADP | 18.1 MRs. |
| Development of Bt transgenic and colored cotton in Pakistan in collaboration with Pak-China. | RADP | 35.46 MRs. |
| 2019 | |
| Ashraf A, Rehman OU, Muzammil S, Léon J, Naz AA, Rasool F, Ali GM, Zafar Y, Khan MR* (2019) Evolution of Deeper Rooting 1-like Homoeologs in Wheat Entails the C-Terminus Mutations as well as Gain and Loss of Auxin Response Elements. PLoS ONE 14(4): e0214145. https://doi.org/10.1371/journal. pone.0214145 | |
| Hussain T, Rehman N, Inam S, Afroz A, Muhammad A, Ajmal W, Zafar Y, Ghulam Muhammad Ali, Khan MR. 2019. Homotypic clusters of transcription factor binding sites in the first large intron of AGL24 MADS-box transcription factor are recruited in the enhancement of floral expression. Plant Molecular Biology Reporter. DOI : 10.1007/s11105-019-01136-7 | |
| 2018 | |
| Waqar, Aqsa, Sahir Hameed Khattak, Sania Begum, Tayyaba Rehman, Armghan Shehzad, Wajya Ajmal, Syeda Shahdana Zia, Iqra Siddiqi, and Ghulam Muhammad Ali, (2018). "Stripe Rust: A Review of the Disease, Yr Genes and its Molecular Markers." Sarhad Journal of Agriculture 34(1). | |
| Muhammad S. Z., Ghulam Muhammad Ali, A. Muhammad and I. Hussain (2018). In vitro Screening of Salt Tolerance in Potato (Solanum tuberosum L.) Varieties. Sarhad Journal of Agriculture, Vol. 56(1): 41-47. | |
| Khan, A., Ali, Ghulam Muhammad Ali, S., Zia, M. A., Jan, S. A., & Um-E-Robab. (2018). Biosafety assessment of genetically engineered wheat (triticum aestivum l.) under greenhouse conditions. Pakistan Journal of Agricultural Sciences. 55(2), 263-270. | |
| Khalid Mehmood, Muhammad Arshad, Ghulam Muhammad Ali, Abdul Razzaq and Rehmatullah Qureshi. (2018). Agrobacterium mediated callus based transformation protocol for transferring DREB1A gene in wheat (Triticum aestivum L.) Journal of Pure and Applied Agriculture 3(1): 33-41 | |
| Ali, Shaukat; Rana, Aamir; Zia, Muhammad Amir; Khan, Siffat Ullah; Iqbal, Arshad; Ali, Ghulam Muhammad Ali. (2018) Temporal status of bt gene expression in Pakistani cotton. Pakistan Journal of Agricultural Sciences. 55 (1) 143-150. | |
| Zia, M. A., Shinwari, Z. K., Ali, S., Shah, S. H., Anwar, M., Ghulam Muhammad Ali. (2018). Comparison among different cotton (Gossypium hirsutum L.) genotypes with respect to morphological, fibre quality attributes and expression analysis of Cry1Ac gene. Journal of Animal and Plant Sciences, 28(3), 819-829. | |
| Rehman, N., Khan MR., Abbas Z., Noor S., Inam S. Ghulam Muhammad Ali. (2018). A Mitogen Activated Protein Kinase Kinase (MAPKK) Recruited in Salt Tolerance in Halophytic Salicornia europaea (Amaranthaceae). Mol. Biotech. (Submitted) | |
| Sarmir khan, Ghulam Muhammad Ali, Sidra Irshad Khan Aziz-ud-Din, Irum Arif Sana Waqas, Shakira Ghazanfar. 2018. Screening of autochthonous Lactic Acid Bacteria as Probiotic for Lactating Buffaloes. The Indian Journal of Animal Research (Under Review: Paper ID: ACCR-101). | |
| Ashtar Khan, Ghulam Muhammad Ali, Shaukat Ali, Muhammad Amir Zia, Sohail Ahmad Jan and Um-E-Robab.2018. Biosafety Assessment of genetically modified wheat under greenhouse conditions. PJAS, 55(2):263-270. | |
| Shaukat Ali, Kiran Kareem, Muhammad Amir Zia, Siffat Ullah Khan, Armghan Shahzad, Jalal-udDin and Ghulam Muhammad Ali. 2018. Expression analysis and introgression of transcriptional factor (DREB1A) in tomato for cold tolerance. Journal of Pure and Applied Agriculture (2018) 3(1): 1-12. | |
| Waqar, A., Khattak, S.H., Begum, S., Rehman, T., Rabia, Shehzad, A., Ajmal, W., Zia, S.S., Siddiqi, I. and Ghulam Muhammad Ali. 2018. Stripe Rust: A Review of the Disease, Yr Genes and its Molecular Markers. Sarhad Journal of Agriculture, 34(3). | |
| Muhammad S. Z., G. M. Ali, A. Muhammad and I. Hussain (2018). In vivo screening of potato (solanum tuberosum l.) Cultivars for saline water tolerance. Sarhad Journal of Agriculture, Vol. 56(1): 41-47. | |
| Noor, S., S. Ali, H. Rehman, F. Ullah, Ghulam Muhammad Ali,. (2018). Comparative study of transgenic (DREB1A) and non-transgenic wheat lines on relative water content, sugar, proline and chlorophyll under drought and salt stresses. Sarhad Journal of Agriculture, 34(4): 986-993. | |
| 2017 | |
| Abbas, Zaheer, Shaukat Ali, and Ghulam M. Ali, (2017). "In Vitro Plant Regeneration from Coleoptilar Node of Maize Seedling: a New Tool to Bioengineer the Maize Rapidly." Sarhad Journal of Agriculture 33(4). | |
| Arif, Shazia, Muhammad Ramzan Khan, Syed Dilnawaz Ahmed Gardezi, Muhammad Fareed Khan, Abdul Hamid, Asad Hussain Shah, and Ghulam Muhammad Ali, (2017). "Molecular Cloning and Transcript Profiling Of Ascorbate Oxidase Gene at Different Growth Developmental Stages From Therapeutically Important Plant Seabuckthorn Hippophae Rhamnoides L." Pakistan Journal of Botany, 49(3): 1143-1154. | |
| Inam, Safeena, Muhammad Ramzan Khan, Nazia Rehman, Zaheer Abbas, Sabahat Noor, and Ghulam Muhammad Ali, (2017). "Molecular Detection and Quantification of Non-Basmati Adulterants in Basmati Rice using BADH2 Gene Marker." International Journal of Agriculture and Biology, 19(6): 1463-1468. | |
| Abbas, Zaheer, Shaukat Ali, and Ghulam M. Ali, (2017). "Optimization of Different Parameters for Transformation of Elite Maize Inbred Lines." Sarhad Journal of Agriculture 33(4). | |
| David J. Spielman, Fatima Zaidi, Patricia Zambrano, Asif Ali Khan, Shaukat Ali, H. Masooma Naseer Cheema, Hina Nazli, Rao Sohail Ahmad Khan, Arshad Iqbal, Muhammad Amir Zia, Ghulam Muhammad Ali, (2017). "What are farmers really planting? Measuring the presence and effectiveness of Bt cotton in Pakistan." PloS one, 12(5): e0176592. | |
| Rehman, Nazia, Muhammad Ramzan Khan, and Ghulam Muhammad Ali, (2017). "A novel phytoene synthase paralog from halophytic Salicornia confers salinity tolerance in plants." Turkish Journal of Botany, 41(2). | |
| Ghaffar, Abdul, Muhammad Naeem Riaz, Ahmad Ali, Aatka Jamil, Tahira Kamal, and Ghulam Muhammad Ali, (2017). "Genotyping of β-lactoglobulin Gene in Pakistani Dairy Cattle (Sahiwal Breed) by Using PCR-RFLP." PSM Veterinary Research 2(2): 10-13. | |
| Hussain, Tanveer, Muneeb Musthafa, Masroor Ellahi Babar, Faiz Marikar, Fiaz Hussain, Saeed Akram Khan, Shahid Sherzada, and Ahmad Ali. "Genomic analysis and comparison of Pakistani camels (Camelus dromedarius) by prion gene, (2017)." Acta Scientiae Veterinariae, 45: 1-8. | |
| Saeed, Shazia, Muhammad Younas Khan Barozai, Alia Ahmed, Rasool Baksh Tareen, And Sania Begum and Ghulam Muhammad Ali. "Impact of Ecological Diversity on Genetic and Phytochemical Variation Injuniperus Excelsa from High Elevation Zones of Quetta Valley, Pakistan." Pak. J. Bot, 49(1): 201-206. | |
| Sabir Hussain Shah, Shaukat Ali,, Abdul Ahad Qureshi, Muhammad Amir , Jalal-ud-Din and Ghulam Muhammad Ali. 2017 Chilling tolerance in three tomato transgenic lines overexpressing CBF3 gene controlled by a stress inducible promoter. Environmental Science and Pollution Research. 24 (22) 18536-18553. | |
| Sidra iqbal, Farhatullah, Malik Ashiq Rabbani, Mahwish Kanwal, Nahida yousaf and Sania Begum. (2017). Investigation and confirmation of introgression in brassica Species using microsatellite markers. Pak. J. Bot., 49(6): 2213-2220. | |
| Zaynab, M., Kanwal, S., Hussain, I., Qasim, M., Noman, A., Iqbal, U., Ghulam Muhammad Ali., Bahadar, K., Jamil, A., Sughra, K., Rehman, N., Buriro, M., Abbas, S., Ali, M., Alvi, A.H., Anwar, M., Khan, M.I., Tayyab, M., 2017. Rice Chitinase Gene Expression in Genetically Engineered Potato Confers Resistance against Fusarium solani and Rhizoctonia solani. PSM Microbiology. 2(3): MB-2017-016. | |
| Zaynab M., Kanwal S., Furqan M., Ghulam Muhammad Ali., Rehman N., Zafar S., Sughra S., Jahanzab M. 2017. Proteomics approach to address low seed germination in Cyclobalnopsis gilva, Biotechnology Letters. 39 (10) 1441-1451. | |
| 2016 | |
| Arif, Shazia, Muhammad Ramzan Khan, Syed Dilnawaz Ahmed Gardezi, Zaheer Abbas, and Ghulam Muhammad Ali, (2016). "A Novel Hydroxymethyldihydropterin Pyrophosphokinase-dihydropteroate Synthase (HPPK-DHPS) Gene from a Nutraceutical Plant Seabuckthorn, Involved in Folate Pathway is Predominantly Expressed in Fruit Tissue." International Journal of Agriculture and Biology, 18(2): 412-418. | |
| Mehmood, Khalid, Muhammad Arshad, Shaukat Ali, Mazher Qayyum, and Ghulam Muhammad Ali, (2016). "Comparative study of tissue culture response of some selected basmati rice cultivars of Pakistan." Journal of Rural Development and Agriculture, 1(1): 30-38. | |
| Shah, Sabir Hussain, Shaukat Ali, Zahid Hussain, Sohail Ahmad Jan, and Ghulam Muhammad Ali, (2016). "Genetic Improvement of Tomato (Solanum lycopersicum) with AtDREB1A Gene for Cold Stress Tolerance using Optimized Agrobacterium-mediated Transformation System." International Journal of Agriculture & Biology, 18(3). | |
| Iqbal, Muhammad, Mahwish Ejaz, Iftikhar Ahmed, Armghan Shahzad, and Ghulam M. Ali, (2016). "Molecular genetic variation for stripe rust resistance in spring wheat." Pakistan Journal of Agricultural Sciences, 53(1). | |
| Rana, Aamir, Syed Sajjad Sattar, Afshann Shahzad, Ghulam Muhammad Ali, and Yasir Waheed, (2016). "Scavenger receptor class-A plays diverse role in innate immunity, cell signaling and different pathologies." Asian Pacific Journal of Tropical Disease, 6(7): 567-572. | |
| Faqir, Nadia, Aish Muhammad, Armghan Shehzad, Muhammad Zeeshan Hyder, and Ghulam Muhammad Ali, (2016). "Simple Sequence Repeat (SSR) markers show greater similarity among morphologically diverse Date palm (Phoenix dactylifera L.) cultivars grown in Pakistan." Pure and Applied Biology, 5(3): 483. | |
| Akram, Zahid, Shaukat Ali, Ghulam Muhammad Ali, Yusuf Zafar, Zahid Hussain Shah, and Fahad Alghabari, (2016). "Whisker-mediated transformation of peanut with chitinase gene enhances resistance to leaf spot disease." Crop Breeding and Applied Biotechnology, 16(2): 108-114. | |
| Khan, Muhammad Ramzan, Humera Ihsan, and Ghulam Muhammad Ali, (2016). "WSA206, a paralog of duplicated MPF2-like MADS-box family is recruited in fertility function in Withania." Plant Science, 253: 215-228. | |
| 2015 | |
| Shah, Sabir Hussain, Saukat Ali, S. A. Jan, J. Din, and Ghulam Muhammad Ali, (2015). "Callus induction, in vitro shoot regeneration and hairy root formation by the assessment of various plant growth regulators in tomato (Solanum lycopersicum Mill.)." Journal of Animal and Plant Sciences, 25: 528-538. | |
| Mahmood, Tariq, Ghulam Muhammad Ali, and Armghan Shehzad, (2015). "Comprehensive overview for developing drought tolerant transgenic wheat (Triticum aestivum L.)." Journal of Agrobiology, 30(2): 55-69. | |
| Aamir Rana, Siffat Ullah Khan, Shaukat Ali, Muhammad Amir Zia, Arshad Iqbal, Ghulam Muhammad Ali and Muhammad Tahir, (2015). Effect of Temperature on Bt Gene Expression in Candidate Cotton Lines of Pakistan American-Eurasian Journal of Toxicological Sciences, 7(1): 26-33. | |
| Noman, Muhammad, Wajya Ajmal, Muhammad Ramzan Khan, Armghan Shahzad, and Ghulam Muhammad Ali, (2015). "Exploitation of Concatenated Olive Plastome DNA Markers for Reliable Varietal Identification for On-Farm Genetic Resource Conservation." American Journal of Plant Sciences, 6(19): 3045. | |
| Saeed, S., Barozai, Y.K., Ahmed, A., Tareen, R.B., Ghulam Muhammad Ali, Shehzad, Armgan. and Begum, Sania., 2015. Genetic diversity of Ephedra procera from high altitudes of Quetta valley, Balochistan using RAPD and ISSR. Pak. J. Weed Sci. Res, 21(2): 163-172. | |
| Zaman, Muhammad Shah, Ghulam Muhammad Ali, Aish Muhammad, Khalid Farooq, and Iqbal Hussain, (2015). "In vitro screening of salt tolerance in potato (Solanum tuberosum L.) varieties." Sarhad Journal of Agriculture, 31(2): 106-113. | |
| Shah, Sabir Hussain, Shaukat Ali, Sohail Ahmad Jan, and Ghulam Muhammad Ali. "Piercing and incubation method of in planta transformation producing stable transgenic plants by overexpressing DREB1A gene in tomato (Solanum lycopersicum Mill.), (2015)." Plant Cell, Tissue and Organ Culture (PCTOC), 120(3): 1139-1157. | |
| Ashraf, S., A. Shahzad, F. Karamat, Muhammad Iqbal, and Ghulam Muhammad Ali, (2015). "Quantitative trait loci (QTLs) analysis of drought tolerance at germination stage in a wheat population derived from synthetic hexaploid and opata, (2015)." Journal of Animal and Plant Sciences, 25(2): 539-545. | |
| Jan, Sohail Ahmad, Sabir Hussain Shah, Shaukat Ali, and Ghulam Muhammad Ali, (2015). "The effect of plant growth regulators on callus induction and somatic embryogenesis of hybrid tomato." Pak. J. Bot, 47(5): 1671-1677. | |
| Ejaz, Mahwish, Muhammad Iqbal, Muhammad Naeemullah, Iftikhar Ahmed, Armghan Shahzad, M. Shahid Masood, and Ghulam Muhammad Ali, (2015). "Validation and use of dna markers for sex determination in papaya (Carica papya)." Pak. J. Bot 47(3): 1051-1059. | |
| Ihsan, Humera, Muhammad Ramzan Khan, Wajya Ajmal, and Ghulam Muhammad Ali, (2015). "WsMAGO2, a duplicated MAGO NASHI protein with fertility attributes interacts with MPF2-like MADS-box proteins." Planta, 241(5): 1173-1187. | |
| Hussain T, Babar ME, Musthafa MM, Saif R, Hussain F, Aqeel M, Naveed N, Pervez MT, Khan WA, Ziaullah, Shahzad S and Yaqub A, (2015). Mitochondrial ATP6 and ATP8 Genes Based Molecular Diversity and Phylogenetic Analysis in Punjab Urial (Ovis vignei punjabiensis). Journal of Animal and Plant Sciences, 25(3 Supp. 2): 311-317. | |
| 2014 | |
| Ahmad Iftikhar and Ghulam Muhammad Ali, (2014). Agricultural Biotechnology Adoption and Biosafety Regulations in Pakistan South Asia Biosafety Program Newsletter. Vol 11. No 1 | |
| Begum, Sania, Muhammad Iqbal, Iftikhar Ahmed, Muhammad Fayyaz, Armghan Shahzad, and Ghulam M. Ali, (2014). "Allelic variation at loci controlling stripe rust resistance in spring wheat." Journal of genetics, 93(2): 579-586. | |
| Shah, Sabir Hussain, Shaukat Ali, Sohail Ahmad Jan, and Ghulam Muhammad Ali, (2014). "Assessment of carbon sources on in vitro shoot regeneration in tomato." Pakistan Journal of Agricultural Sciences, 51(1). | |
| Shah, Sabir Hussain, SHAUKAT Ali, SOHAIL AHMAD Jan, J. Din, and Ghulam Muhammad Ali, (2014). "Assessment of silver nitrate on callus induction and in vitro shoot regeneration in tomato (Solanum lycopersicum Mill.)." Pakistan Journal of Botany, 46(6): 2163-2172. | |
| 2013 | |
| Rana, Aamir, Ghulam M. Ali, Shaukat Ali, Ammara Khan, Sajid Mansoor, Shazia Malik, and Ammad A. Farooqi, (2013). "BCR-ABL1 in leukemia: Disguise master outplays riding shotgun." Journal of cancer research and therapeutics, 9(1): 6. | |
| Khan, Muhammad Ramzan, and Ghulam Muhammad Ali, (2013). "Functional evolution of cis-regulatory modules of STMADS11 superclade MADS-box genes." Plant molecular biology, 83(4-5): 489-506. | |
| Rana, Aamir, Ghulam M. Ali, Shaukat Ali, Ammara Khan, Bibi Sabiha, Shazia Malik, Asma M. Riaz, and Ammad A. Farooqi, (2013). "Breakpoint cluster region-c-abl oncogene 1, non-receptor tyrosine kinase signaling: Current patterns of the versatile regulator revisited." Journal of cancer research and therapeutics, 9(1): 3. | |
| Mehmood, KHALID., MUHAMMAD Arshad, Ghulam Muhammad Ali, and Abdul Razzaq, (2013). "Tissue culture responses of some wheat (Triticum aestivum L.) cultivars grown in Pakistan." Pakistan Journal of Botany 45: 545-549. | |
| Sabir Hussain Shah, Saukat Ali, and Ghulam Muhammad Ali, (2013). "A novel approach for rapid in vitro morphogenesis in tomato (Solanum lycopersicum Mill.) with the application of cobalt chloride." European Academic Research, 1: 2702-2721. | |
| Ali, Niaz, Muhammad Iqbal, Iftikhar Ahmed, and Muhammad Asif, (2013). "Genetic variation at loci controlling quality traits in spring wheat." Pakistan Journal of Agricultural Sciences, 50(4). | |
| Kumar, Tanweer, Muhammad Ramzan Khan, Zaheer Abbas, and Ghulam Muhammad Ali, (2013). "Genetic improvement of sugarcane for drought and salinity stress tolerance using Arabidopsis vacuolar pyrophosphatase (AVP1) gene." Molecular biotechnology, 56(3): 199-209. | |
| Iqbal, Arshad, Shaukat Ali, Muhammad Amir Zia, Armghan Shahzad, Jalal Ud Din, Ullah Asad, Muhammad Asad, Ghulam Muhammad Ali, and Yusuf Zafar, (2013). "Comparative Account of Bt Gene Expression in Cotton under Normal and Salt Affected Soil." International Journal of Agriculture & Biology, 15(6). | |
| 2012 | |
| Ejaz, Mahwish, Muhammad Iqbal, Armghan Shahzad, Iftikhar Ahmed, and Ghulam Muhammad Ali, (2012). "Genetic variation for markers linked to stem rust resistance genes in Pakistani wheat varieties." Crop Science, 52(6): 2638-2648. | |
| Muhammad Ramzan Khan, I. Khan, and Ghulam Muhammad Ali, (2012). "MPF2-like MADS-box genes affecting SOC1 and MAF1 expression are implicated in flowering time control." Molecular Biotechnology, (12): 9540-9549. | |
| Shaukat Ali, Sabir Hussain Shah, Ghulam Muhammad Ali, Arshad Iqbal, Muhammad Asad Ullah Asad, and Yusuf Zafar, (2012). "Bt Cry toxin expression profile in selected Pakistani cotton genotypes." Current Science, 102(12): 1632-1636. | |
| Ahmad, Muhammad Zulfiqar, Iqbal Hussain, Aish Muhammad, Shaukat Ali, Ghulam Muhammad Ali, Sohaib Roomi, Muhammad Amir Zia, and Amir Ijaz, (2012). "Factor affecting Agrobacterium-mediated transformation of rice chitinase gene in Solanum tuberosum L." African Journal of Biotechnology, 11(41): 9716-9723. | |
| Khan, Muhammad Ramzan, Jinyong Hu, and Ghulam Muhammad Ali, (2012). "Reciprocal loss of CArG-boxes and auxin response elements drives expression divergence of MPF2-Like MADS-box genes controlling calyx inflation." PLoS One, 7(8): e42781. | |
| Armgan Shahzad, M. Ahmad, M. Iqbal, I. Ahmed, and Ghulam Muhammad Ali, (2012). "Evaluation of wheat landrace genotypes for salinity tolerance at vegetative stage by using morphological and molecular markers." Genetics and Molecular Research, 11(1): 679-692. | |
| Uzma, Muhammad Ramzan Khan, Aish Muhammad, Iqbal Hussain, Sabir Hussain Shah, Tanweer Kumar, Safeena Inam, Muhammad Zubair, Hafeez-Ur-Rehman, Altaf Sher, Nazia Rehman, Sajeela Ahmed And Ghulam Muhammad Ali, (2012). Rapid in vitro multiplication of sugarcane elite genotypes and detection of sugarcane mosaic virus through two steps RT-PCR. International Journal of Agriculture and Biology, 14(6): 870-878. | |
| Iqbal, Muhammad Munir, Farhat Nazir, Shaukat Ali, M. Ahsan Asif, Yusuf Zafar, Javaid Iqbal, and Ghulam Muhammad Ali, (2012). "Over expression of rice chitinase gene in transgenic peanut (Arachis hypogaea L.) improves resistance against leaf spot." Molecular biotechnology, 50(2): 129-136. | |
| 2011 | |
| Afroz, Amber, Ghulam Muhammad Ali, Asif Mir, and Setsuko Komatsu, (2011). "Application of proteomics to investigate stress-induced proteins for improvement in crop protection." Plant cell reports, 30(5): 745-763. | |
| Rana, Aamir, Shahzad Bhatti, Ghulam Muhammad Ali, Shoukat Ali, Nazia Rehman, Sabir Hussain Shah, and Ammad Ahmad Farooqi, (2011). "Authentication of fusion genes in chronic myeloid leukemia." International Journal of Biotechnology and Molecular Biology Research, 2(7): 128-130. | |
| Rana, Aamir, Sabir Hussain Shah, Nazia Rehman, Shaukat Ali, Shahzad Bhatti, and Ammad Ahmad Farooqi, (2011). "Chronic myeloid leukemia: Attributes of break point cluster region-abelson (BCR-ABL)." Journal of Cancer Research and Experimental Oncology, 3(6): 62-66. | |
| Afroz, Amber, Ghulam Muhammad Ali, Asif Mir, and Setsuko Komatsu, (2011). "Application of proteomics to investigate stress-induced proteins for improvement in crop protection." Plant cell reports, 30(5): 745-763. | |
| Iqbal, Muhammad, Muhammad Fayyaz, Armghan Shahzad, Iftikhar Ahmed, Ghulam Muhammad Ali, Shahid Masood, and Dean Spaner, (2011). "Effect of vernalization on grain fill duration And grain weight in spring wheat." Pak. J. Bot, 43(1): 689-694. | |
| Asif, Muhammad Ahsan, Yusuf Zafar, Javaid Iqbal, Muhammad Munir Iqbal, Umer Rashid, Ghulam Muhammad Ali, Anjuman Arif, and Farhat Nazir, (2011). "Enhanced expression of AtNHX1, in transgenic groundnut (Arachis hypogaea L.) improves salt and drought tolerence." Molecular biotechnology, 49(3): 250-256. | |
| Nazir, Farhat, Zahid Akram, Muhammad Matloob Javed, Shoukat Ali, Ghulam Muhammad Ali, and Yusuf Zafar, (2011). "In vitro regeneration of Pakistani peanut (Arachis hypogea L.) varieties using de-embryonated coteledonary explants." African Journal of Biotechnology, 10(43): 8599-8604. | |
| Afroz, Amber, Zubeda Chaudhry, Umer Rashid, Ghulam Muhammad Ali, Farhat Nazir, Javaid Iqbal, and Muhammad Rashid Khan, (2011). "Enhanced resistance against bacterial wilt in transgenic tomato (Lycopersicon esculentum) lines expressing the Xa21 gene." Plant Cell, Tissue and Organ Culture (PCTOC), 104(2): 227-237. | |
| 2010 | |
| Ali, Shaukat, Yusuf Zafar, Ghulam Muhammad Ali, and Farhat Nazir, (2010). "Bacillus thuringiensis and its application in agriculture." African Journal of Biotechnology, 9(14): 2022-2031. | |
| Ali, Shaukat, Shahid Hameed, Shahid Masood, Ghulam Muhammad Ali, and Yusuf Zafar, (2010). "Status of Bt cotton cultivation in major growing areas of Pakistan." Pak. J. Bot, 42(3): 1583-1594. | |
| Afroz, Amber, Zubeda Chaudhry, Umer Rashid, Muhammad Rashid Khan, and Ghulam Muhammad Ali, (2010). "Enhanced regeneration in explants of tomato (Lycopersicon esculentum L.) with the treatment of coconut water." African Journal of Biotechnology, 9(24): 3634-3644. | |
| Munazir, Mehmooda, Rahmatullah Qureshi, Ghulam Muhammad Ali, Umer Rashid, Sabahat Noor, Khalid Mehmood, Shoukat Ali, and Muhammad Arshad, (2010). "Primary callus induction, somatic embryogenesis and regeneration studies in selected elite wheat varieties from Pakistan." Pak. J. Bot, 42(6): 3957-3965. | |
| 2009 | |
| Rashid, Umer, Shaukat Ali, Ghulam Muhammad Ali, Najma Ayub, and M. Shahid Masood, (2009). "Establishment of an efficient callus induction and plant regeneration system in Pakistani wheat (Triticum aestivum) cultivars." Electronic Journal of Biotechnology, 12(3): 4-5. | |
| Noor, Sabahat, Ghulam Muhammad Ali, Umer Rashid, Muhammad Arshad, Shoukat Ali, and Yusuf Zafar, (2009). "Optimization of callus induction and regeneration system for Pakistani wheat cultivars Kohsar and Khyber-87." African Journal of Biotechnology, 8(20). | |
| 2008 | |
| Ali, Shaukat, Yusuf Zafar, Zhang Xianyin, Ghulam Muhammad Ali, and Tu Jumin, (2008). "Transgenic crops: Current challenges and future perspectives." African Journal of Biotechnology, 7(25). |
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Contact Us
For further queries, feel free to contact
National Institute for Genomics and Advanced Biotechnology - NIGAB
National Agrictural Research Center - NARC
Park Road, Chack Shehzad Islamabad - Pakistan
Tel: 92-51-90733812